RESUMO
Resumen El Centro de Protección e Higiene de las Radiaciones fue fundado en 1985 para sustentar la aplicación segura de las tecnologías nucleares en Cuba. En la actualidad la institución es considerada como una referencia nacional y regional en materia de seguridad radiológica, gracias a una sólida estrategia institucional que aporta de manera sostenida soluciones a problemas prioritarios del país. Este trabajo presenta una reseña de los principales resultados del centro hasta la fecha.
Abstract The Center for Radiation Protection and Hygiene was founded in 1985 to support the safe application of nuclear technologies in Cuba. Nowdays, the institution is considered as a national and regional reference for radiation protection , thanks to the comprehensive institutional strategy that support the solutions of the problems with priority in the country. The paper presents the overview about the main results of the center up today.
RESUMO
Purpose: This paper describes how the BioDoseNet image repository was used as a training tool for the dicentric assay.Materials and methods: The training was implemented in three phases: introduction to dicentric scoring, dose response curve elaboration and dose assessment exercise. Four labs without previous experience in the dicentric assay participated and four modules of the repository were used.Results: The labs become familiar with aberrations induced by ionizing radiation. The labs were able to generate data for the elaboration of a dose response curve and then successfully estimated doses and irradiated fractions in six blind samples.Conclusions: The performance of these laboratories during the exercise demonstrates the efficacy of the BioDoseNet image repository as a training tool and the utility of web based scoring for the dicentric assay community.
Assuntos
Aberrações Cromossômicas/efeitos da radiação , Análise Citogenética , Bases de Dados Factuais , Imagem Molecular , Calibragem , Relação Dose-Resposta à Radiação , Metáfase/genética , Metáfase/efeitos da radiaçãoRESUMO
PURPOSE: This paper presents a calibration curve for prematurely condensed chromosome rings (PCC-ring) which are scarce in cytogenetic dosimetry; here we analyzed the behavior of the PCC-ring frequency and PCC index after gamma irradiation in the dose interval from 0 to 20 Gy. MATERIALS AND METHODS: PCC-rings were induced in lymphocytes with Calyculin A. A total of 5788 PCC cells in G1, G2/M, and M/A stages were analyzed. RESULTS: The best fitting curve between the frequency of PCC ring (Y) and the Dose (D) was obtained with the equation Y = (0.0308 ± 0.0012) D. The distribution of rings by cell followed a Poisson distribution. The PCC index was correlated with radiation dose and decreased in a dose-dependent manner. CONCLUSION: This PCC-ring dose-effect curve can be used in case of high dose accidental overexposure to gamma radiation, allowing a dose assessment in a reliable way in most of the simulated irradiation analyzed.
Assuntos
Aberrações Cromossômicas/efeitos da radiação , Raios gama/efeitos adversos , Calibragem , Relação Dose-Resposta à Radiação , HumanosRESUMO
El ozono es un poderoso agente oxidante. Sin embargo, en predeterminadas condiciones y concentraciones es aplicado en enfermedades que cursan con un déficit en las defensas antioxidantes con el objetivo de estimularlas. Para el estudio del efecto genotóxico del ozono gaseoso utilizado en la autohemoterapia se estableció un diseño experimental el cual fue el realizado en 3 pacientes tratados con 200 mL de O3/O2(50 mg/mL) vía autohemoterapia y 1 paciente tratado vía rectal con la misma dosis y concentración. Se tomaron 0,5 ml de sangre antes y después del tratamiento en los días 1, 7 y 14 y se manifestó un incremento del daño en los días 1 y 14 de la terapia después del tratamiento, no ocurriendo así en el día 7, donde se reflejó un posible período de adaptación. En ambos casos el análisis de las muestras se llevó a cabo utilizando el ensayo alcalino de electroforesis de células individuales o ensayo cometa que evalúa las rupturas de simple cadena y los sitios labiles al álcali en el ADN(AU)
